Once you have paid for something via debit card online will your bank stop you from taking that money out?

• online merchant account
• online merchant services
• paid online services

I've just booked a hotel and bought train tickets and my balance says 190 but I can only take 70 out does that mean its safe to take the 70 pound out and it won't affect getting my tickets etc?

Once you have paid for something via debit card online will your bank stop you from taking that money out?